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- Document 3219
- DOCN M94A3219
- TI Modulation of Th1 responses in HIV infection and tuberculosis (TB).
- DT 9412
- AU Barnes P; Zhang M; Jones B; Univ. of Southern California, Los Angeles.
- SO Int Conf AIDS. 1994 Aug 7-12;10(1):126 (abstract no. PA0122). Unique
- Identifier : AIDSLINE ICA10/94369356
- AB To evaluate the Th1/Th2 cytokine profile in HIV-infected TB patients, we
- evaluated proliferative responses to M. tb and patterns of cytokine
- production by PBMC from 40 TB patients, with or without concomitant HIV
- infection. Upon stimulation with M. tb in vitro, PBMC from HIV-infected
- TB patients had reduced proliferation (mean delta cpm 1989 versus 26110,
- p < 0.0001) and production of IFN-gamma (mean 822 versus 2278 pg/ml, p =
- 0.004), but IL-10 production was not diminished (mean 711 versus 578
- pg/ml). We next used RT-PCR with internal controls to quantitate
- cytokine mRNA in M. tb-stimulated PBMC from TB patients, 12 with and 13
- without HIV infection. Samples were normalized to contain 0.4 attoM of
- CD3 delta chain cDNA, then amplified by PCR with cytokine-specific
- primers. mRNA for the Th1 cytokines IFN-gamma and IL-2 was significantly
- reduced in HIV-infected TB patients (mean .069 versus .544 attoM of
- IFN-gamma cDNA, and .003 versus .025 attoM of IL-2 cDNA). In contrast,
- mRNA for the Th2 cytokines IL-4 and IL-10 was comparable in TB patients
- with or without HIV infection. In purified CD4+ cells, mRNA for Th1
- cytokines was still lower in HIV-infected patients. M. tb-induced
- proliferative responses by PBMC from HIV-infected TB patients were
- markedly enhanced by addition of anti-IL-10 in 10/11 patients, and by
- addition of recombinant IL-12 in 6/10 patients. Proliferation was not
- enhanced by anti-IL-4. M. tb-induced production of IFN-gamma was
- markedly enhanced by anti-IL-10 or IL-12. We conclude that Th1 responses
- are reduced in HIV-infected TB patients, and that these responses can be
- augmented by antibodies to IL-10 or by IL-12.
- DE Antibodies/PHARMACOLOGY AIDS-Related Opportunistic
- Infections/*IMMUNOLOGY Cells, Cultured Comparative Study
- Cytokines/*BIOSYNTHESIS DNA Primers Human Interferon Type
- II/BIOSYNTHESIS Interleukin-10/ANTAGONISTS & INHIB/BIOSYNTHESIS
- Interleukin-2/BIOSYNTHESIS Interleukin-4/ANTAGONISTS & INHIB
- Interleukins/PHARMACOLOGY Lymphocyte Transformation/DRUG EFFECTS
- Lymphocytes/DRUG EFFECTS/*IMMUNOLOGY Polymerase Chain Reaction
- Recombinant Proteins/PHARMACOLOGY RNA, Messenger/BIOSYNTHESIS
- Tuberculosis/*IMMUNOLOGY MEETING ABSTRACT
-
- SOURCE: National Library of Medicine. NOTICE: This material may be
- protected by Copyright Law (Title 17, U.S.Code).
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-